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Expression Pattern Data Submission Form :

 Please fill out as many fields as possible. First two fields are required.

REQUIRED
Submitter's Name : e.g. Diana Chen => Name
Submitter's Email : e.g. dchen@nowhere.com => Email
If you don't get a verification email, email us at webmaster@wormbase.org
What is Expressed :
DNA Sequence :
(for all types of experiments)
Confirmed Gene : e.g. egl-10, abc-1 => Confirmed Gene
Predicted Gene : e.g. B0261.2 => Predicted Gene
Detailed Sequence : e.g. from nt 14006 to nt 14225 from cosmid C52E4.6 => Detailed Sequence
Protein Sequence :
(for immunostaining or Western)
Peptide Sequence
(for antibody preparation) :		 e.g. NFHGDSISFSFSSA => Peptide Sequence
Other Peptide : e.g. GST-tagged recombinant protein ABC-1 protein expressed from E. Coli => Other Peptide
Where is the gene Expressed :
Tissue Distribution :
Summary (Tissue) : e.g. Expressed in HSN, male-specific nervous system and intestine at adult stage. Expressed in AB.p at 4-cell embryo ... => Summary (Tissue)
Cell : e.g. HSNL, HSNR, AB.p => Cell
Tissue : e.g. male-specific nervous system, intestine => Tissue
Life Stage : e.g. adult, embryo (see http... for Life Stage Ontology) => Life Stage
Subcellular Localization :
Summary (Subcellular) : e.g. Expressed in cytoplasm ... => Summary (Subcellular)
How were the experiments conducted :
Reporter_gene : e.g. [abc-1::gfp] and [abc-1::LacZ] translational constructs were created by inserting nt 12345 - nt 12567 from cosmid A1B2 into pPD95.63 ... => Reporter_gene
Immunostaining : e.g. Polyclonal Rabbit peptide antibody was created by injecting peptide NFHGDSISFSFSSA into rabbit ... => Immunostaining
In_situ : e.g. Using abc-1 cDNA yk12b3 as sense probe ... => In_situ
Northern : e.g. Using 0.9kb BamH1/HindIII fragment from abc-1 cDNA yk12b3 as probe ... => Northern
Western : e.g. Polyclonal Rabbit peptide antibody was created by injecting peptide NFHGDSISFSFSSA into rabbit ... => Western
RT-PCR : e.g. Using oligo A1 "ATCGGGACCTTGGC" and A2 "GGCGGTTAGCTATAGC" => RT-PCR
Mammalian Cell Culture : e.g. Using COS7 cell transfection. Expression construct is created by ... Expression pattern was identified using antibody ... => Mammalian Cell Culture
Other approach : Indicate here what it is. => Other approach
Data Source :
Published Data :
Reference ID : e.g. CGC5509, PMID13452789, wcwm2002p356, wbg17p8, ... => Reference ID
Reference Info : e.g. RYN Lee et all 2002, Development 157, 1234-1245 ... => Reference Info
Personal Data :
Pers. Comm. : e.g. Personal Communication from Diana Chen, Dec 1, 2002 => Pers. Comm.
If you have any problems, questions, or comments, please contact draciti@its.caltech.edu